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Reduction of the canonical function of a glycolytic enzyme enolase triggers immune responses that further affect metabolism and growth in Arabidopsis.
Yang, Leiyun; Wang, Zhixue; Zhang, Aiqin; Bhawal, Ruchika; Li, Chunlong; Zhang, Sheng; Cheng, Lailiang; Hua, Jian.
Afiliação
  • Yang L; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
  • Wang Z; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
  • Zhang A; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
  • Bhawal R; Proteomics and Metabolomics Facility, Cornell University, New York 14853, USA.
  • Li C; Horticulture Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
  • Zhang S; Proteomics and Metabolomics Facility, Cornell University, New York 14853, USA.
  • Cheng L; Horticulture Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
  • Hua J; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA.
Plant Cell ; 34(5): 1745-1767, 2022 04 26.
Article em En | MEDLINE | ID: mdl-34791448
ABSTRACT
Primary metabolism provides energy for growth and development as well as secondary metabolites for diverse environmental responses. Here we describe an unexpected consequence of disruption of a glycolytic enzyme enolase named LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 2 (LOS2) in causing constitutive defense responses or autoimmunity in Arabidopsis thaliana. The autoimmunity in the los2 mutant is accompanied by a higher expression of about one-quarter of intracellular immune receptor nucleotide-binding leucine-rich repeat (NLR) genes in the genome and is partially dependent on one of these NLR genes. The LOS2 gene was hypothesized to produce an alternatively translated protein c-Myc Binding Protein (MBP-1) that functions as a transcriptional repressor. Complementation tests show that LOS2 executes its function in growth and immunity regulation through the canonical enolase activity but not the production of MBP-1. In addition, the autoimmunity in the los2 mutants leads to a higher accumulation of sugars and organic acids and a depletion of glycolytic metabolites. These findings indicate that LOS2 does not exert its function in immune responses through an alternatively translated protein MBP-1. Rather, they show that a perturbation of glycolysis from the reduction of the enolase activity results in activation of NLR-involved immune responses which further influences primary metabolism and plant growth, highlighting the complex interaction between primary metabolism and plant immunity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2022 Tipo de documento: Article