CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages.

Doran, Ciara G; Sugisawa, Ryoichi; Carty, Michael; Roche, Fiona; Fergus, Claire; Hokamp, Karsten; Kelly, Vincent P; Bowie, Andrew G

Doran, Ciara G; Sugisawa, Ryoichi; Carty, Michael; Roche, Fiona; Fergus, Claire; Hokamp, Karsten; Kelly, Vincent P; Bowie, Andrew G.

Afiliação

Doran CG; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Sugisawa R; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Carty M; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Roche F; School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, Ireland.
Fergus C; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Hokamp K; School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, Ireland.
Kelly VP; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Bowie AG; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. Electronic address: agbowie@tcd.ie.

J Biol Chem ; 297(6): 101417, 2021 12.

Article em En | MEDLINE | ID: mdl-34793837

Assuntos

Proteínas do Domínio Armadillo; Sistemas CRISPR-Cas; Proteínas do Citoesqueleto; Epitopos; Regulação da Expressão Gênica; Macrófagos/metabolismo; Transcrição Gênica; Animais; Proteínas do Domínio Armadillo/biossíntese; Proteínas do Domínio Armadillo/genética; Proteínas do Citoesqueleto/biossíntese; Proteínas do Citoesqueleto/genética; Epitopos/genética; Epitopos/metabolismo; Camundongos; Camundongos Knockout; Neurônios/metabolismo; Vincristina/metabolismo

Palavras-chave

CRISPR/Cas9; SARM1; axon degeneration; chemokines; innate immunity; passenger genes; transcription

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Regulação da Expressão Gênica / Proteínas do Citoesqueleto / Proteínas do Domínio Armadillo / Sistemas CRISPR-Cas / Macrófagos / Epitopos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google