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Quantitative Off-Target Detection of Epstein-Barr Virus-Derived DNA in Routine Molecular Profiling of Hematopoietic Neoplasms by Panel-Based Hybrid-Capture Next-Generation Sequencing.
Petrova-Drus, Kseniya; Quesada, Andrés E; Bowman, Anita S; Ptashkin, Ryan; Yao, Jinjuan; Arcila, Maria E; Ho, Caleb; Moung, Christine; Regalado, Jennifer; Benayed, Ryma; Benhamida, Jamal K; Galera, Pallavi K; Dogan, Ahmet; Vanderbilt, Chad.
Afiliação
  • Petrova-Drus K; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York. Electronic address: petrovak@mskcc.org.
  • Quesada AE; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Bowman AS; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Ptashkin R; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Yao J; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Arcila ME; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Ho C; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Moung C; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Regalado J; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Benayed R; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Benhamida JK; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Galera PK; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Dogan A; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Vanderbilt C; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
J Mol Diagn ; 24(1): 69-78, 2022 01.
Article em En | MEDLINE | ID: mdl-34801704
Epstein-Barr virus (EBV) is associated with hematologic and solid tumors. We utilized a hybridization capture-based next-generation sequencing (NGS) platform targeting 400 genes associated with hematological malignancies to detect and quantify nontargeted viral-derived EBV reads that aligned to the EBV reference contig (NC_007605). We evaluated 5234 samples from 3636 unique patients with hematological neoplasms and found that 100 samples (1.9%) in 93 unique patients had ≥6 EBV reads (range, 6 to 32,325; mean, 827.5; median, 54). Most (n = 73, 73%) represented known EBV-associated conditions, and the most common was post-transplant lymphoproliferative disorders (n = 21, 29%). Documented EBV viremia was found in 4 of 27 samples with a moderate quantity of EBV reads and conditions not known to be EBV associated, whereas suspected viremia or low-level activation was likely in the remaining 23 samples. A good correlation (Spearman r = 0.8; 95% CI, 0.74-0.85) was found between EBV reads by NGS and systematic semiquantitative EBV-encoded RNA in situ hybridization in 162 available samples, particularly at greater EBV involvement. An optimal threshold for significant morphologic EBV involvement was found to be ≥10 reads by the receiver operating characteristic analysis (area under the curve, 0.990; 95% CI, 0.9974%-1.000%). Thus, in addition to mutational analysis, hybrid-capture-based NGS panels can detect and quantitate off-target EBV-derived viral DNA, which correlates well with morphology.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Hematológicas / Infecções por Vírus Epstein-Barr Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Hematológicas / Infecções por Vírus Epstein-Barr Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article