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A Rapid and Simple Multiparameter Assay to Quantify Spike-Specific CD4 and CD8 T Cells after SARS-CoV-2 Vaccination: A Preliminary Report.
Shekarkar Azgomi, Mojtaba; La Manna, Marco Pio; Badami, Giusto Davide; Ragonese, Paolo; Trizzino, Antonino; Dieli, Francesco; Caccamo, Nadia.
Afiliação
  • Shekarkar Azgomi M; Central Laboratory of Advanced Diagnostic and Biomedical Research (CLADIBIOR), University of Palermo, 90127 Palermo, Italy.
  • La Manna MP; Department of Biomedicine, Neurosciences and Advanced Diagnostic (Bi.N.D.), University of Palermo, 90127 Palermo, Italy.
  • Badami GD; Central Laboratory of Advanced Diagnostic and Biomedical Research (CLADIBIOR), University of Palermo, 90127 Palermo, Italy.
  • Ragonese P; Department of Biomedicine, Neurosciences and Advanced Diagnostic (Bi.N.D.), University of Palermo, 90127 Palermo, Italy.
  • Trizzino A; Central Laboratory of Advanced Diagnostic and Biomedical Research (CLADIBIOR), University of Palermo, 90127 Palermo, Italy.
  • Dieli F; Department of Biomedicine, Neurosciences and Advanced Diagnostic (Bi.N.D.), University of Palermo, 90127 Palermo, Italy.
  • Caccamo N; Department of Biomedicine, Neurosciences and Advanced Diagnostic (Bi.N.D.), University of Palermo, 90127 Palermo, Italy.
Biomedicines ; 9(11)2021 10 29.
Article em En | MEDLINE | ID: mdl-34829805
mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4+ and CD8+ T cell responses induced by vaccination in healthy donors and in subjects with B cell compart impairment, in which antibody response is absent due to primary immunodeficiencies or CD20 depleting therapy. We detect and quantified memory T cell immune responses against SARS-CoV-2 evocated by vaccination in both groups, irrespective to the humoral response. Furthermore, we identified TNF-α as the main cytokine produced by T memory cells, after antigen-specific stimulation in vitro, that could be considered, other than IFN-γ, an additional biomarker of induction of T memory cells upon vaccination. Further studies on the vaccine-induced T cell responses could be crucial, not only in healthy people but also in immunocompromised subjects, where antigen specific T cells responses play a protective role against SARS-CoV-2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article