In Vivo Cellular-Level 3D Imaging of Peripheral Nerves Using a Dual-Focusing Technique for Intra-Neural Interface Implantation.
Adv Sci (Weinh)
; 9(3): e2102876, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34845862
ABSTRACT
In vivo volumetric imaging of the microstructural changes of peripheral nerves with an inserted electrode could be key for solving the chronic implantation failure of an intra-neural interface necessary to provide amputated patients with natural motion and sensation. Thus far, no imaging devices can provide a cellular-level three-dimensional (3D) structural images of a peripheral nerve in vivo. In this study, an optical coherence tomography-based peripheral nerve imaging platform that employs a newly proposed depth of focus extension technique is reported. A point spread function with the finest transverse resolution of 1.27 µm enables the cellular-level volumetric visualization of the metal wire and microstructural changes in a rat sciatic nerve with the metal wire inserted in vivo. Further, the feasibility of applying the imaging platform to large animals for a preclinical study is confirmed through in vivo rabbit sciatic nerve imaging. It is expected that new possibilities for the successful chronic implantation of an intra-neural interface will open up by providing the 3D microstructural changes of nerves around the inserted electrode.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Isquiático
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Imageamento Tridimensional
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Tomografia de Coerência Óptica
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Eletrodos Implantados
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article