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Intranasal HD-Ad vaccine protects the upper and lower respiratory tracts of hACE2 mice against SARS-CoV-2.
Cao, Huibi; Mai, Juntao; Zhou, Zhichang; Li, Zhijie; Duan, Rongqi; Watt, Jacqueline; Chen, Ziyan; Bandara, Ranmal Avinash; Li, Ming; Ahn, Sang Kyun; Poon, Betty; Christie-Holmes, Natasha; Gray-Owen, Scott D; Banerjee, Arinjay; Mossman, Karen; Kozak, Rob; Mubareka, Samira; Rini, James M; Hu, Jim; Liu, Jun.
Afiliação
  • Cao H; Translational Medicine Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada.
  • Mai J; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Zhou Z; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Li Z; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Duan R; Translational Medicine Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada.
  • Watt J; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Chen Z; Translational Medicine Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada.
  • Bandara RA; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Li M; Translational Medicine Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada.
  • Ahn SK; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Poon B; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Christie-Holmes N; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Gray-Owen SD; Combined Containment Level 3 Unit, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Banerjee A; Combined Containment Level 3 Unit, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Mossman K; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Kozak R; Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
  • Mubareka S; Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.
  • Rini JM; Department of Biology, University of Waterloo, Waterloo, ON, Canada.
  • Hu J; Department of Medicine Institute for Infectious Disease Research, McMaster Immunology Research Center, McMaster University, Hamilton, ON, Canada.
  • Liu J; Sunnybrook Heath Sciences Centre, Toronto, ON, Canada.
Cell Biosci ; 11(1): 202, 2021 Dec 08.
Article em En | MEDLINE | ID: mdl-34879865
ABSTRACT

BACKGROUND:

The ongoing COVID-19 pandemic has resulted in 185 million recorded cases and over 4 million deaths worldwide. Several COVID-19 vaccines have been approved for emergency use in humans and are being used in many countries. However, all the approved vaccines are administered by intramuscular injection and this may not prevent upper airway infection or viral transmission.

RESULTS:

Here, we describe a novel, intranasally delivered COVID-19 vaccine based on a helper-dependent adenoviral (HD-Ad) vector. The vaccine (HD-Ad_RBD) produces a soluble secreted form of the receptor binding domain (RBD) of the SARS-CoV-2 spike protein and we show it induced robust mucosal and systemic immunity. Moreover, intranasal immunization of K18-hACE2 mice with HD-Ad_RBD using a prime-boost regimen, resulted in complete protection of the upper respiratory tract against SARS-CoV-2 infection.

CONCLUSION:

Our approaches provide a powerful platform for constructing highly effective vaccines targeting SARS-CoV-2 and its emerging variants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article