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Viral Agents and Systemic Levels of Inflammatory Cytokines in Vulnerable and Stable Atherosclerotic Carotid Plaques.
Mazzaccaro, Daniela; Dolci, Maria; Perego, Federica; Delbue, Serena; Giannetta, Matteo; Cardani, Rosanna; Valentina Renna, Laura; Costa, Elena; Corsi-Romanelli, Massimiliano M; Galli, Cristina; Pariani, Elena; Nano, Giovanni; Clemente, Claudio; Basilico, Nicoletta.
Afiliação
  • Mazzaccaro D; Operative Unit of Vascular Surgery, IRCCS Policlinico San Donato, Milan, Italy. Electronic address: danymazzak83@libero.it.
  • Dolci M; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
  • Perego F; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
  • Delbue S; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
  • Giannetta M; Operative Unit of Vascular Surgery, IRCCS Policlinico San Donato, Milan, Italy.
  • Cardani R; BioCor Biobank, UO of Clinical Pathology, Department of Pathology and Laboratory Medicine, IRCCS-Policlinico San Donato, Milan, Italy.
  • Valentina Renna L; BioCor Biobank, UO of Clinical Pathology, Department of Pathology and Laboratory Medicine, IRCCS-Policlinico San Donato, Milan, Italy.
  • Costa E; UO of Clinical Pathology, Department of Pathology and Laboratory Medicine, IRCCS-Policlinico San Donato, Milan, Italy.
  • Corsi-Romanelli MM; UO of Clinical Pathology, Department of Pathology and Laboratory Medicine, IRCCS-Policlinico San Donato, Milan, Italy; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
  • Galli C; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
  • Pariani E; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
  • Nano G; Operative Unit of Vascular Surgery, IRCCS Policlinico San Donato, Milan, Italy; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
  • Clemente C; UO SMEL-2, Surgical Pathology, Department of Pathology and Laboratory Medicine, IRCCS-Policlinico San Donato, Milan, Italy.
  • Basilico N; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
Ann Vasc Surg ; 82: 325-333, 2022 May.
Article em En | MEDLINE | ID: mdl-34902464
BACKGROUND: To investigate the presence of genetic material of viral agents and the serum level of inflammatory cytokines in patients submitted to carotid endarterectomy having vulnerable versus stable atherosclerotic plaques. METHODS: Data of patients consecutively submitted to carotid endarterectomy for a significant stenosis from July 2019 to December 2019 were prospectively collected. The genetic material of Epstein-Barr (EBV), CitoMegalo (CMV), Herpes Simplex (HSV), Varicella-Zoster (VZV) and Influenza (IV) Viruses was searched in the patient's plaques, both in the "mid" of the plaque and in an adjacent lateral portion of no-plaque area. The serum levels of TNF-α, IL-1ß, IL-6, IL10 and CCL5 were determined. The obtained results were then correlated to the histologic vulnerability of the removed carotid plaque. P values < 0.05 were considered statistically significant. RESULTS: Data of 50 patients were analyzed. A vulnerable plaque was found in 31 patients (62%). The genome of CMV, HSV, VZV and IV was not found in any of the vascular samples, while the EBV genome was found in the "mid" of 2 vulnerable plaques, but not in their respective control area. Eighty-two percent of patients who did not receive anti-IV vaccination (23/28) had vulnerable carotid plaque, compared with 36% of vaccinated patients (8/22, P = 0.001). Serum levels of TNF-α and IL-6 were higher in patients with a vulnerable plaque compared to patients with a stable plaque (73.6 ± 238.2 vs. 3.9 ± 13.1 pg/ml, P= 0.01, and 45.9 ± 103.6 vs. 10.1 ± 25.3 pg/ml, P= 0.01, respectively), independent of comorbidities, viral exposure or flu vaccination. CONCLUSIONS: The EBV genome was found in the "core" of 2 vulnerable carotid plaques, but not in their respective adjacent control. Influenza vaccination was associated with a lower incidence of carotid plaque vulnerability. Serum levels of TNF-α and IL-6 were higher in patients with a vulnerable plaque compared to patients with a stable plaque.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Interleucina-6 / Fator de Necrose Tumoral alfa / Endarterectomia das Carótidas / Estenose das Carótidas / Infecções por Citomegalovirus / Placa Aterosclerótica Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Interleucina-6 / Fator de Necrose Tumoral alfa / Endarterectomia das Carótidas / Estenose das Carótidas / Infecções por Citomegalovirus / Placa Aterosclerótica Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article