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Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system.
Canac, Robin; Caillaud, Amandine; Cimarosti, Bastien; Girardeau, Aurore; Hamamy, Hanan; Reversade, Bruno; Bonnard, Carine; Al Sayed, Zeina R; David, Laurent; Poschmann, Jeremie; Lemarchand, Patricia; Lamirault, Guillaume; Gaborit, Nathalie.
Afiliação
  • Canac R; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Caillaud A; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Cimarosti B; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Girardeau A; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Hamamy H; Department of Genetic Medicine and Development, Geneva University, Geneva 1211, Switzerland.
  • Reversade B; Genome Institute of Singapore, A*STAR, Singapore, Singapore; Medical Genetics Department, Koç University School of Medicine (KUSOM), Istanbul, Turkey; Department of Paediatrics, National, University of Singapore, Singapore, Singapore; Institute of Molecular and Cell Biology, A*STAR, Singapore, Singa
  • Bonnard C; Skin Research Institute of Singapore, A*STAR, Singapore, Singapore.
  • Al Sayed ZR; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • David L; Université de Nantes, CHU Nantes, INSERM, CNRS, SFR Santé, FED 4203, INSERM UMS 016, CNRS, UMS 3556, F-44000 Nantes, France; Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France.
  • Poschmann J; Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France.
  • Lemarchand P; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Lamirault G; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.
  • Gaborit N; Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France. Electronic address: nathalie.gaborit@univ-nantes.fr.
Stem Cell Res ; 58: 102627, 2022 01.
Article em En | MEDLINE | ID: mdl-34929443
ABSTRACT
Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article