Significance of cytochrome P-450 (P-450 HFLa) of human fetal livers in the steroid and drug oxidations.
Biochem Pharmacol
; 36(4): 453-6, 1987 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-3493777
ABSTRACT
The purpose of this study was to clarify the pharmacological and physiological significance of P-450 HFLa. Thus, correlations between cytochrome P-450 (P-450 HFLa) level and different monooxygenase activities were investigated in liver homogenates from human fetuses. Poor correlation was seen between P-450 HFLa level and the activity of benzphetamine N-demethylation or aniline hydroxylation. In contrast, the content of P-450 HFLa was highly correlated with the activity of benzo(a)pyrene hydroxylation, 7-ethoxycoumarin O-deethylation or testosterone 6 beta-hydroxylation. In microsomes from human adult livers, a moderate relationship was also observed between testosterone 6 beta-hydroxylation and P-450 HFLa level. Furthermore, antibodies to P-450 HFLa inhibited testosterone 6 beta-hydroxylase activity in fetal and adult livers to similar extents. We conclude that P-450 HFLa is a form of cytochrome P-450 which catalyzes testosterone 6 beta-hydroxylation and limited drug oxidations in human fetal and adult livers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrocarboneto de Aril Hidroxilases
/
Sistema Enzimático do Citocromo P-450
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Isoenzimas
/
Fígado
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article