Chimeric crRNA improves CRISPR-Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2.

Yang, Jun; Barua, Nilakshi; Rahman, Md Nannur; Lo, Norman; Tsang, Tsz Fung; Yang, Xiao; Chan, Paul K S; Zhang, Li; Ip, Margaret

Yang, Jun; Barua, Nilakshi; Rahman, Md Nannur; Lo, Norman; Tsang, Tsz Fung; Yang, Xiao; Chan, Paul K S; Zhang, Li; Ip, Margaret.

Afiliação

Yang J; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Barua N; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Rahman MN; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Lo N; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Tsang TF; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Yang X; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Chan PKS; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.
Zhang L; Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Hong Kong (SAR), China.
Ip M; Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China.

PLoS One ; 16(12): e0261778, 2021.

Article em En | MEDLINE | ID: mdl-34941928

Assuntos

COVID-19/diagnóstico; Técnicas de Amplificação de Ácido Nucleico/métodos; SARS-CoV-2/genética; COVID-19/genética; Sistemas CRISPR-Cas/genética; Primers do DNA/genética; Testes Diagnósticos de Rotina/métodos; Humanos; Mutação/genética; RNA Guia de Cinetoplastídeos/genética; RNA Guia de Cinetoplastídeos/metabolismo; Sensibilidade e Especificidade

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Amplificação de Ácido Nucleico / SARS-CoV-2 / COVID-19 Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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