Genome-Wide Analysis of DNA Methylation in Buccal Cells of Children Conceived through IVF and ICSI.
Genes (Basel)
; 12(12)2021 11 28.
Article
em En
| MEDLINE
| ID: mdl-34946866
ABSTRACT
Early life periconceptional exposures during assisted reproductive technology (ART) procedures could alter the DNA methylation profiles of ART children, notably in imprinted genes and repetitive elements. At the genome scale, DNA methylation differences have been reported in ART conceptions at birth, but it is still unclear if those differences remain at childhood. Here, we performed an epigenome-wide DNA methylation association study using Illumina InfiniumEPIC BeadChip to assess the effects of the mode of conception on the methylome of buccal cells from 7- to 8-year-old children (48 children conceived after ART or naturally (control, CTL)) and according to the embryo culture medium in which they were conceived. We identified 127 differentially methylated positions (DMPs) and 16 differentially methylated regions (DMRs) (FDR < 0.05) with low delta beta differences between the two groups (ART vs. CTL). DMPs were preferentially located inside promoter proximal regions and CpG islands and were mostly hypermethylated with ART. We highlighted that the use of distinct embryo culture medium was not associated with DNA methylation differences in childhood. Overall, we bring additional evidence that children conceived via ART display limited genome-wide DNA methylation variation compared with those conceived naturally.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fertilização in vitro
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Genoma Humano
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Ilhas de CpG
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Metilação de DNA
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Injeções de Esperma Intracitoplásmicas
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Técnicas de Reprodução Assistida
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Mucosa Bucal
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Child
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article