BLI-MS: Combining biolayer interferometry and mass spectrometry.
Proteomics
; 22(9): e2100031, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-34958708
ABSTRACT
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Interferometria
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article