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Integrin CD11b provides a new marker of pre-germinal center IgA+ B cells in murine Peyer's patches.
Gao, Peng; Adachi, Takahiro; Okai, Shinsaku; Morita, Naoki; Kitamura, Daisuke; Shinkura, Reiko.
Afiliação
  • Gao P; Institute for Quantitative Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
  • Adachi T; Graduate School of Frontier Science, University of Tokyo, Kashiwa-shi, Chiba 277-8561, Japan.
  • Okai S; Department of Precision Health, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062, Japan.
  • Morita N; Department of Applied Immunology, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
  • Kitamura D; Institute for Quantitative Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
  • Shinkura R; Division of Cancer Biology, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Noda, Chiba 278-0022, Japan.
Int Immunol ; 34(5): 249-262, 2022 04 20.
Article em En | MEDLINE | ID: mdl-34971392
ABSTRACT
Activated B cells can enter germinal centers (GCs) for affinity maturation to produce high-affinity antibodies. However, which activated B cells will enter GCs remains unknown. Here, we found a small population of CD11b+IgA+ B cells located outside of GCs in murine Peyer's patches (PPs). After injection of the CD11b+IgA+ PP B cells into a PP of a recipient mouse, they entered GCs forty hours later. They expressed GC surface markers and pre-GC B cell genes, suggesting that CD11b provides a novel surface marker of pre-GC IgA+ B cells in murine PPs. Furthermore, independently of dendritic cell activation, CD11b expression on B cells can be induced by bacterial antigens, such as pam3CSK4 and heat-killed Escherichia coli in vitro. In addition, mice orally administered with pam3CSK4 or heat-killed E. coli increased the number of PP GC B cells within two days, and enhanced the mucosal antigen-specific IgA response. Our results demonstrate that the induction of CD11b on B cells is a promising marker for selecting an effective mucosal vaccine adjuvant.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nódulos Linfáticos Agregados / Integrinas / Antígeno CD11b Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nódulos Linfáticos Agregados / Integrinas / Antígeno CD11b Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article