Extraction of Metabolites from Cancer Cells.
Methods Mol Biol
; 2445: 329-335, 2022.
Article
em En
| MEDLINE
| ID: mdl-34973001
ABSTRACT
Cancer cells possess an elevated demand for nutrients and metabolites due to their uncontrolled proliferation and need to survive in unfavorable conditions. Autophagy is a conservative degradation pathway that counters lack of nutrients and provides organelle and protein quality control, beyond maintenance of cellular metabolism.Mass spectrometry-based metabolomics is a powerful tool to study the metabolome of a cell. Such analysis requires proper sample preparation including the extraction of metabolites. Here, we provide a protocol for the extraction of metabolites from adherent cancer cells suitable for global metabolome profiling by mass spectrometry.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Metaboloma
/
Neoplasias
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article