The effects of nicotine, cotinine and anabasine on rat adrenal 11 beta-hydroxylase and 21-hydroxylase.
J Steroid Biochem
; 28(1): 25-8, 1987 Jul.
Article
em En
| MEDLINE
| ID: mdl-3497305
ABSTRACT
The effects of nicotine, cotinine and anabasine on rat adrenal steroidogenesis were examined by spectral and enzymatic techniques. The addition of nicotine, cotinine or anabasine to preparations of rat adrenal mitochondria produced type II cytochrome P-450 difference spectra. The addition of nicotine or anabasine, but not cotinine, to rat adrenal microsomes yielded type II cytochrome P-450 difference spectra. Nicotine and anabasine competitively inhibited rat adrenal mitochondrial 11 beta-hydroxylase and microsomal 21-hydroxylase. Cotinine competitively inhibited mitochondrial 11 beta-hydroxylase, but did not inhibit microsomal 21-hydroxylase. The apparent enzymatic inhibition constants for cotinine, nicotine, anabasine and metyrapone inhibition of the mitochondrial 11 beta-hydroxylase were 32, 96, 120 and 74 microM respectively. These studies suggest that components of cigarette smoke may alter patterns of adrenal steroidogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piperidinas
/
Pirrolidinonas
/
Esteroide Hidroxilases
/
Esteroide 11-beta-Hidroxilase
/
Esteroide 21-Hidroxilase
/
Glândulas Suprarrenais
/
Cotinina
/
Anabasina
/
Nicotina
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article