Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.
STAR Protoc
; 3(1): 101056, 2022 03 18.
Article
em En
| MEDLINE
| ID: mdl-35005638
ABSTRACT
Host tRNAs specifically interact with the matrix domain (MA) of HIV-1 major structural polyprotein, Gag, to control its membrane localization and virion assembly. In this protocol, we describe the purification and engineering of HIV-1 MA and tRNA, and the co-crystallization and structure determination of the complex using X-ray crystallography. Rational engineering of the tRNA surface created tRNA-tRNA packing contacts that drove the formation of diffraction-quality co-crystals. This protocol can be adapted to solve other ribonucleoprotein complex structures containing structured RNAs. For complete details on the use and execution of this protocol, please refer to Bou-Nader et al. (2021).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
HIV-1
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article