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Analysis of a Cancer Stem Cell-Derived Single Colony Raised in a Microwell Array.
Lei, Kin Fong; Chen, Qing-En; Goh, Andrew; Pai, Ping Ching.
Afiliação
  • Lei KF; Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan.
  • Chen QE; Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou, Taoyuan 333, Taiwan.
  • Goh A; Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan.
  • Pai PC; Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan.
ACS Appl Bio Mater ; 4(6): 5099-5105, 2021 06 21.
Article em En | MEDLINE | ID: mdl-35007058
Cancer stem cells (CSCs) were reported to play important roles in cancer initialization, progression, and metastasis. In order to study the variation between CSCs and non-CSCs, single-cell analysis is conducted but technically complicated. In the current work, a microwell array made by an agarose hydrogel was developed for the study of a CSC-derived single colony. This approach is simple, convenient, and compatible with the setting and skill set of existing biological laboratories. Single cells, double cells, and multiple cells were distributed in the microwells. Isolation of CSCs could be achieved after a 5 day starvation culture course. Then, a CSC-derived single colony was formed by culturing the CSCs in a nutritious culture medium for another 5 days. The results revealed that a single CSC presented a high colony formation rate. Multiple cells containing CSCs and non-CSCs could raise a larger single colony than the multiple cells with CSCs only. Although CSCs possess an aggressive characteristic, development of a solid tumor requires the proactive involvement of non-CSCs. This work showed a practical demonstration of using a microwell array for the investigation of a CSC-derived single colony.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Neoplasias Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Neoplasias Idioma: En Ano de publicação: 2021 Tipo de documento: Article