FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver.
STAR Protoc
; 3(1): 101070, 2022 03 18.
Article
em En
| MEDLINE
| ID: mdl-35024628
ABSTRACT
Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24low progenitors are less mature and have greater proliferative capacity than CD71/CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24low progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Precursoras Eritroides
/
Eritropoese
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article