Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica.
Int J Syst Evol Microbiol
; 72(1)2022 Jan.
Article
em En
| MEDLINE
| ID: mdl-35044904
ABSTRACT
A Gram-stain-positive, aerobic, non-sporulating, yellow-pigmented and rod or cocci-shaped bacterium, designated Arc0846-15T, was isolated from the kelp Laminaria japonica. Strain Arc0846-15T was found to grow at 16-35 °C (optimum, 28 °C), at pH 6.0-9.5 (optimum, 7.0) and in the presence of 0-6â% (w/v) NaCl (optimum, 2â%). Cells were positive for catalase and negative for oxidase activity. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, revealed that the nearest phylogenetic neighbour strains of strain Arc0846-15T were Ornithinimicrobium murale 01 Gi-040T (96.2â%), Ornithinimicrobium kibberense K22-20T (96.1â%) and Ornithinimicrobium humiphilum HKI 0124T (95.2â%). Based on phylogenomic analysis, the average nucleotide identity values between strain Arc0846-15T and the neighbour strains were 69.8, 69.7 and 69.8â%, respectively; the digital DNA-DNA hybridization values between strain Arc0846-15T and its three closest neighbour strains were 18.8, 19.1 and 19.3â%, respectively. The predominant menaquinone was MK-8 (H4). The dominant cellular fatty acids were C17ââ1 ω8c, iso-C15ââ0, iso-C16ââ0 and C17ââ0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain Arc0846-15T was 61.6 mol% based on the whole genome sequence. Based on the phylogenetic and phenotypic characteristics, strain Arc0846-15T is considered to represent a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium laminariae sp. nov. is proposed, with Arc0846-15T (=KCTC 49655T=MCCC 1K06093T) as the type strain.
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01-internacional
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MEDLINE
Assunto principal:
Filogenia
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Actinobacteria
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Kelp
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Laminaria
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article