One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system.

Raman, Rajeev; Karpova, Anna; Kreutz, Michael R

Raman, Rajeev; Karpova, Anna; Kreutz, Michael R.

Afiliação

Raman R; Research Group Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany. Electronic address: rajeev.raman@lin-magdeburg.de.
Karpova A; Research Group Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany; Center for Behavioral Brain Sciences, Otto von Guericke University, 39120, Magdeburg, Germany.
Kreutz MR; Research Group Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany; Center for Behavioral Brain Sciences, Otto von Guericke University, 39120, Magdeburg, Germany; Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany; German Center for Neurodegenerative Diseases (DZNE), 39120, Magdeburg, Germany.

Protein Expr Purif ; 193: 106057, 2022 05.

Article em En | MEDLINE | ID: mdl-35077781

Assuntos

Escherichia coli; Lamina Tipo B; Escherichia coli/genética; Escherichia coli/metabolismo; Lamina Tipo B/química; Lamina Tipo B/metabolismo

Palavras-chave

Intein-tag; Lamin B1; Large scale purification; Lyophilisation; Polymerization

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lamina Tipo B / Escherichia coli Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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