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Development of bone cell microarrays in microfluidic chips for studying osteocyte-osteoblast communication under fluid flow mechanical loading.
Yvanoff, Charlotte; Willaert, Ronnie G.
Afiliação
  • Yvanoff C; Research Group Structural Biology Brussels, Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Vrije Universiteit Brussel, Brussels, Belgium.
  • Willaert RG; International Joint Research Group VUB-EPFL (Lausanne, Switzerland) NanoBiotechnology and NanoMedicine (NANO), Vrije Universiteit Brussel, Brussels, Belgium.
Biofabrication ; 14(2)2022 02 22.
Article em En | MEDLINE | ID: mdl-35108702
ABSTRACT
Bone tissue remodels throughout life in response to mechanical loads. Impaired activities of bone cells (osteocytes, osteoblasts and osteoclasts) result in a disruption of the bone remodelling cycle, which eventually leads to bone disorders such as osteoporosis. To develop efficient therapeutic strategies against bone disorders, new tools are needed to unravel the bone remodelling cycle at the molecular level. Here, we developed a microfluidic platform, which should allow understanding the bone remodelling cycle in much more detail and ultimately be used to discover new therapeutic compounds. We focused specifically on studying cell-cell communication between osteocytes and osteoblasts cells via connexin 43-gap junctions. Therefore, a new cell printing method was developed to create living cellular bone cell arrays in a microfluidic channel. Several cell printing designs where osteocytes and osteoblasts heterotypically interacted at localized interfaces were evaluated. Physical contacts between the bone cells were characterized at high resolution by correlative atomic force microscopy (AFM)-fluorescence microscopy. We demonstrated that the platform is compatible with single-cell mechanostimulation by AFM nanoindentation and subsequent fluorescent analysis of the mechanoresponse. As a proof of concept, we showed the functionality of the platform by analysing the inducedin vivo-like Ca++wave in the printed osteocyte-osteoblast network upon mechanical stimulation by fluid flow shear stress.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteócitos / Microfluídica Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteócitos / Microfluídica Idioma: En Ano de publicação: 2022 Tipo de documento: Article