Zebrafish integrin a3b is required for cardiac contractility and cardiomyocyte proliferation.
Biochem Biophys Res Commun
; 595: 89-95, 2022 03 05.
Article
em En
| MEDLINE
| ID: mdl-35121232
ABSTRACT
In cardiac muscle cells, heterodimeric integrin transmembrane receptors are known to serve as mechanotransducers, translating mechanical force to biochemical signaling. However, the roles of many individual integrins have still not been delineated. In this report, we demonstrate that Itga3b is localized to the sarcolemma of cardiomyocytes from 24 to 96 hpf. We further show that heterozygous and homozygous itga3b/bdf mutant embryos display a cardiomyopathy phenotype, with decreased cardiac contractility and reduced cardiomyocyte number. Correspondingly, proliferation of ventricular and atrial cardiomyoctyes and ventricular epicardial cells is decreased in itga3b mutant hearts. The contractile dysfunction of itga3b mutants can be attributed to cardiomyocyte sarcomeric disorganization, including thin myofilaments with blurred and shortened Z-discs. Together, our results reveal that Itga3b localizes to the myocardium sarcolemma, and it is required for cardiac contractility and cardiomyocyte proliferation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Proteínas de Peixe-Zebra
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Miócitos Cardíacos
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Integrina alfa3
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Miocárdio
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article