Destabilization of DNA and RNA G-quadruplex structures formed by GGA repeat due to N6-methyladenine modification.
Biochem Biophys Res Commun
; 597: 134-139, 2022 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-35144176
ABSTRACT
N6-methyladenine (m6A) is the most abundant RNA modification in eukaryotic RNA. Further, m6A has been identified in the genomic DNA of both eukaryotes and prokaryotes. The G-quadruplex (G4) structure is a non-canonical nucleic acid structure formed by the stacking of GGGG tetrads. In this study, we evaluated the effect of m6A modifications on G4 structures formed by GGA repeat oligonucleotides, d(GGA)8, d(GGA)4, and r(GGA)4. The d(GGA)8 forms an intramolecular tetradheptadheptadtetrad G4 structure, while d(GGA)4 forms a dimerized intermolecular tetradheptadheptadtetrad G4 structure. r(GGA)4 forms a dimerized intermolecular tetradhexadhexadtetrad G4 structure. Circular dichroism melting analysis demonstrated that (1) m6A modifications destabilized the G4 structure formed by d(GGA)8, (2) m6A modification at A3 disrupted the G4 structure formed by d(GGA)4, and (3) m6A modification at A3 destabilized the G4 structure formed by r(GGA)4. m6A modifications may be involved in controlling G4 structure formation to regulate biological functions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article