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Spatial organization of transcribed eukaryotic genes.
Leidescher, Susanne; Ribisel, Johannes; Ullrich, Simon; Feodorova, Yana; Hildebrand, Erica; Galitsyna, Alexandra; Bultmann, Sebastian; Link, Stephanie; Thanisch, Katharina; Mulholland, Christopher; Dekker, Job; Leonhardt, Heinrich; Mirny, Leonid; Solovei, Irina.
Afiliação
  • Leidescher S; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Ribisel J; Institute for Medical Engineering and Science, and Department of Physics, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Ullrich S; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Feodorova Y; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Hildebrand E; Department of Medical Biology, Medical University of Plovdiv; Division of Molecular and Regenerative Medicine, Research Institute at Medical University of Plovdiv, Plovdiv, Bulgaria.
  • Galitsyna A; Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
  • Bultmann S; Skolkovo Institute of Science and Technology, Skolkovo, Russia.
  • Link S; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Thanisch K; BioMedizinisches Center, Ludwig-Maximilians University Munich, Planegg-Martinsried, Germany.
  • Mulholland C; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Dekker J; Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss, Germany.
  • Leonhardt H; Department of Biology II, Biozentrum, Ludwig-Maximilians University Munich (LMU), Planegg-Martinsried, Germany.
  • Mirny L; Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
  • Solovei I; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Nat Cell Biol ; 24(3): 327-339, 2022 03.
Article em En | MEDLINE | ID: mdl-35177821
ABSTRACT
Despite the well-established role of nuclear organization in the regulation of gene expression, little is known about the reverse how transcription shapes the spatial organization of the genome. Owing to the small sizes of most previously studied genes and the limited resolution of microscopy, the structure and spatial arrangement of a single transcribed gene are still poorly understood. Here we study several long highly expressed genes and demonstrate that they form open-ended transcription loops with polymerases moving along the loops and carrying nascent RNAs. Transcription loops can span across micrometres, resembling lampbrush loops and polytene puffs. The extension and shape of transcription loops suggest their intrinsic stiffness, which we attribute to decoration with multiple voluminous nascent ribonucleoproteins. Our data contradict the model of transcription factories and suggest that although microscopically resolvable transcription loops are specific for long highly expressed genes, the mechanisms underlying their formation could represent a general aspect of eukaryotic transcription.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Cromossomos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Cromossomos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article