Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx.
Artif Organs
; 46(7): 1268-1280, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-35191556
ABSTRACT
BACKGROUND:
Hypopharynx reconstruction after hypopharyngectomy is still a great challenge. Perfusion decellularization is for extracellular matrix (ECM) scaffolding and had been used in organ reconstruction. Our study aimed to prepare an acellular, natural, three-dimensional biological hypopharynx with vascular pedicle scaffold as the substitute materials to reconstruct hypopharynx.RESULT:
Scanning electron microscope and histology staining showed that the decellularized hypopharynx with vascular pedicle scaffold retained intact native anatomical ECM structure. Myoblasts were observed on the recellularized scaffolds with bone marrow mesenchymal stem cells induced by 5-azacytidine implanted in the rabbit greater omentum by immunohistochemical analysis.CONCLUSION:
The decellularized hypopharynx with vascular pedicle scaffold prepared by detergent perfusion in our study has a potential to be an alternative material to pharynx reconstruction.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alicerces Teciduais
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article