Methods for isolating and analyzing physiological hyaluronan: a review.
Am J Physiol Cell Physiol
; 322(4): C674-C687, 2022 04 01.
Article
em En
| MEDLINE
| ID: mdl-35196167
ABSTRACT
The carbohydrate hyaluronan (or hyaluronic acid, HA) is found in all human tissues and biofluids where it has wide-ranging functions in health and disease that are dictated by both its abundance and size. Consequently, hyaluronan evaluation in physiological samples has significant translational potential. Although the analytical tools and techniques for probing other biomolecules such as proteins and nucleic acids have become standard approaches in biochemistry, those available for investigating hyaluronan are less well established. In this review, we survey methods related to the assessment of native hyaluronan in biological specimens, including protocols for separating it from biological matrices and technologies for determining its concentration and molecular weight.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Hialuronatos
/
Ácido Hialurônico
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article