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A protocol for full-rotation soft X-ray tomography of single cells.
Chen, Jian-Hua; Vanslembrouck, Bieke; Loconte, Valentina; Ekman, Axel; Cortese, Mirko; Bartenschlager, Ralf; McDermott, Gerry; Larabell, Carolyn A; Le Gros, Mark A; Weinhardt, Venera.
Afiliação
  • Chen JH; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Vanslembrouck B; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Loconte V; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Ekman A; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Cortese M; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Bartenschlager R; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA.
  • McDermott G; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Larabell CA; Department of Infectious Diseases, Molecular Virology Heidelberg University, Heidelberg 69120, Germany.
  • Le Gros MA; Department of Infectious Diseases, Molecular Virology Heidelberg University, Heidelberg 69120, Germany.
  • Weinhardt V; German Center for Infection Research, Heidelberg Partner Site, Heidelberg 69120, Germany.
STAR Protoc ; 3(1): 101176, 2022 03 18.
Article em En | MEDLINE | ID: mdl-35199039
ABSTRACT
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tomografia por Raios X / Imageamento Tridimensional Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tomografia por Raios X / Imageamento Tridimensional Idioma: En Ano de publicação: 2022 Tipo de documento: Article