Effects of breeds and dietary nutrient levels on expression patterns of paired box genes and myogenic regulatory factors in pigs.
Arch Anim Nutr
; 75(6): 474-488, 2021 Dec.
Article
em En
| MEDLINE
| ID: mdl-35227137
ABSTRACT
Two experiments were conducted to investigate the effects of different breeds and dietary nutrient levels on expressions of paired box (Pax) genes and myogenic regulatory factors (MRFs) in pigs. Thirty Large White (LW) barrows and thirty Chenghua (CH, a native breed of China) barrows were performed in experiment 1. Results exhibited that in the CH pigs the abundances of Pax3 at 105 and 220 d of age, Mrf4 at 63 d of age, Myf5 and Mrf4 at 220 d of age were higher than those in the LW pigs (p < 0.05). Meanwhile, the expressions of MyHC-Ð and ÐÐa in the CH pigs were upregulated, and the abundance of MyHC-ÐÐb were downregulated compared with those in the LW pigs at 105 and 220 d of age (p < 0.05). Moreover, the meat quality of the CH pigs was better than in the LW pigs (p < 0.05). In experiment 2, sixty LW pigs were randomly assigned to two dietary treatments meeting their nutrient requirements (NRC) or a diet with moderately reduced digestible energy, crude protein and Lys level by 560 kJ/kg, 1.48% and 0.34%, respectively (LOW diet). The results showed that the reduced dietary nutrient level increased (p < 0.05) the expressions of MyoG and Mrf4 at 105 d of age, Pax3, Myf5, and Mrf4 at 220 d of age, and upregulated (p < 0.05) the abundance of MyHC-ÐÐa at 105 and 220 d of age in LW pigs. In addition, a decrease in dietary nutrient level improved the meat quality in LW pigs (p < 0.05). Collectively, the expressions of Pax genes and MRFs were markedly different between the CH and LW pigs. The CH pigs exhibited higher expression levels of Pax3, Myf5, Mrf4, MyHC-Ð and ÐÐa, which may improved the meat quality. A decrease in dietary nutrient level upregulated the abundances Pax3, Mrf4, Myf5, MyoG, and MyHC-ÐÐa, and might enhance the meat quality in the LW pigs.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dieta
/
Ração Animal
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article