Dimethoxytriadinylation LC-MS/MS of Novichok A-Series Degradation Products in Human Urine.
Anal Chem
; 94(11): 4658-4665, 2022 03 22.
Article
em En
| MEDLINE
| ID: mdl-35253439
ABSTRACT
Novichok A-series compounds, novel nerve agents, pose an increasing threat to citizens worldwide; however, no analytical methods have been reported for detecting their hydrolysis products. Herein, a screening method was developed to detect and identify Novichok A-series degradation products (hydrolysates of A230, A232, A234, A262, and one related compound) and alkyl methylphosphonic acids (RMPAs, conventional nerve agent hydrolysates) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified a suitable derivatization reagent, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM), and optimized the reaction conditions. The derivatized esters of Novichok A-series degradation products were stable and easily detected. We used this derivatization to achieve the first analytical method for Novichok hydrolysis products in urine (0.40-4.0 ng/mL). The detection limits of the RMPAs (0.1-0.4 ng/mL) were comparable to those presented in previous reports involving pentafluorobenzylation or direct LC-MS/MS. The applicability of the newly developed method was evaluated by analyzing urine samples from the OPCW Fifth Biomedical Proficiency Test.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas em Tandem
/
Agentes Neurotóxicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article