A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development.
Commun Biol
; 5(1): 212, 2022 03 08.
Article
em En
| MEDLINE
| ID: mdl-35260793
ABSTRACT
Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Transporte Proteico
/
Descoberta de Drogas
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article