Mesenchymal Stem Cells for Treatment of Delayed-Healing Tympanic Membrane Perforations Using Hyaluronate-based Laminas as a Delivery System: An Animal Model With Histopathologic Study.
Otol Neurotol
; 43(4): e497-e506, 2022 04 01.
Article
em En
| MEDLINE
| ID: mdl-35287155
ABSTRACT
HYPOTHESIS:
Bone marrow derived-mesenchymal stem cells (BM-MSCs) improve the healing of chronic tympanic membrane perforations (cTMPs) in an animal model.BACKGROUND:
cTMPs generate significant morbidity and reduced quality of life, usually requiring surgical assistance. With growing interest in alternative therapies, we sought to evaluate the effect of BM-MSC-therapy on the healing of cTMPs.METHODS:
Sixty cTMPs were established in C57Bl/6 mice and randomized into four groups hyaluronate scaffold as graft plus BM-MSCs (nâ=â19 ears), scaffold plus cell culture media (nâ=â16), scaffold plus phosphate-buffered saline (PBS, nâ=â12), and no intervention (nâ=â13). Hyaluronate scaffolds with or without BM-MSCs were applied on 8-week perforated eardrums. After a blinded assessment of perforation sizes at baseline and 2âweeks after treatment, mean perforation reduction rates (%) were compared. Histology characterization was then performed.RESULTS:
Mean perforation size reduction rates were significantly higher for cTMPs that received scaffolds plus BM-MSCs (Student's t test, pâ=â0.0207, 12.3% [95% CI 7.8-16.7]) and scaffolds plus cell culture media (pâ=â0.0477, 11.3% [95% CI 4.4-18.2]) when compared with no intervention (4.2% [95% CI 1.2-7.2]). This was not observed when treating eardrums with scaffolds plus PBS (7.3% [95% CI 2.7-11.9]). On histology, BM-MSC-treated eardrums demonstrated restoration of the trilaminar configuration and reduced inflammatory changes, while other groups developed tissue architecture disorganization and hypercellular infiltrates surrounding the perforation site.CONCLUSIONS:
BM-MSCs and cell culture media equivalently increased cTMP healing rates. Cell-based therapy conferred a restoration of the trilaminar configuration of the eardrum with relatively compact and organized fibrous layers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfuração da Membrana Timpânica
/
Células-Tronco Mesenquimais
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article