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LINC00936/microRNA-221-3p Regulates Tumor Progression in Ovarian Cancer by Interacting with LAMA3.
Shu, Chenggan; Wang, Weiwei; Wu, Lipei; Qi, Chunrun; Yan, Wenhui; Lu, Wenying; Tian, Jiale; Shang, Anquan.
Afiliação
  • Shu C; Department of Gynecology, The First People's Hospital of Yancheng, City, Yancheng 224006, P.R. China.
  • Wang W; Department of Laboratory Medicine, Shanghai Tongji Hospital, Shanghai, 200065, P.R. China.
  • Wu L; Department of Pathology, Tinghu People's Hospital, Yancheng, 224001, P.R. China.
  • Qi C; Department of Laboratory Medicine, Dongtai People's Hospital & Dongtai Hospital of Nantong University, Yancheng, 224200, P.R. China.
  • Yan W; Department of Pathology, Tinghu People's Hospital, Yancheng, 224001, P.R. China.
  • Lu W; Department of Laboratory Medicine, Tinghu People's Hospital, Yancheng, 224001, P.R. China.
  • Tian J; Department of Laboratory Medicine, Tinghu People's Hospital, Yancheng, 224001, P.R. China.
  • Shang A; Department of Laboratory Medicine, Shanghai Tongji Hospital, Shanghai, 200065, P.R. China.
Article em En | MEDLINE | ID: mdl-35297352
BACKGROUND: Ovarian cancer remains a leading cause of mortality in women. It is known that long non-coding RNA (lncRNA) controls various biological processes and pathogenesis of many diseases, including cancers. This study aimed to determine whether LINC00936 and microRNA-221-3p (miR-221-3p) influence the laminin alpha 3 chain gene (LAMA3) in the development of ovarian cancer. METHODS: The expressions of LINC00936, miR-221-3p, and LAMA3 in ovarian cancer and adjacent tissues were assessed. Furthermore, ovarian cancer cells were transfected with vectors with overexpressed LINC00936, miR-221-3p mimic, miR-221-3p inhibitor, and si-LAMA3 to elucidate their functions in ovarian cancer cell proliferation, migration, invasion, angiogenesis, and tumorigenesis. The binding relationship between LINC00936 and miR-221-3p and the relationship between miR-221-3p and LAMA3 were verified to explore the mechanism of action of LINC00936 in ovarian cancer. LINC00936 binds to miR-221-3p as a ceRNA and regulates the expression of LAMA3. RESULTS: LINC00936 and LAMA3 were poorly expressed, while miR-221-3p was highly expressed in ovarian cancer tissues. Over-expression of LINC00936 contributed to decreasing miR- 221-3p expression and increasing LAMA3 expression. LINC00936 overexpression or miR-221- 3p silencing downregulated the levels of PCNA, MMP-2, MMP-9, and VEGF and decreased cell proliferation, migration, invasion, angiogenesis, and ovarian cancer tumorigenesis. CONCLUSION: Collectively, overexpression of LINC00936 suppressed the development of ovarian cancer by competitively binding to miR-221-3p and controlling LAMA3 expression. These results could serve as a novel theoretical base for the treatment of ovarian cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / MicroRNAs Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / MicroRNAs Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article