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Temporal analysis of paracetamol-induced hepatotoxicity.
Coelho, Aline Meireles; Queiroz, Isabela Ferreira; Lima, Wanderson Geraldo; Talvani, André; Perucci, Luiza Oliveira; Oliveira de Souza, Melina; Costa, Daniela Caldeira.
Afiliação
  • Coelho AM; Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Queiroz IF; Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Lima WG; Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Talvani A; Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Perucci LO; Center for Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Oliveira de Souza M; Department of Food (DEALI), School of Nutrition, Universidade Federal de Ouro Preto (UFOP), Minas Gerais, Brazil.
  • Costa DC; Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Minas Gerais, Brazil.
Drug Chem Toxicol ; 46(3): 472-481, 2023 May.
Article em En | MEDLINE | ID: mdl-35313777
Paracetamol-induced hepatotoxicity (APAP) causes severe damage that may be irreversible. Understanding the evolution of liver injury caused by overdose of the drug is important to assist in the treatment. In the present study, we evaluated the acute intoxication by APAP (500 mg/kg) in periods of 3 and 12 hours in C57BL/6 mice through biochemical, histological, inflammatory parameters, and the redox status. The results showed that in the 3-hour period there was an increase in creatinine dosage and lipid peroxidation (TBARS) compared to the control group. In the period of 12 hours after APAP intoxication all parameters evaluated were altered; there was an increase of ALT, AST, and necrosis, besides the increase of redox status biomarkers as carbonylated protein, TBARS, and MMP-9. We also observed activation of the inflammasome pathway as well as a reduction in the regenerative capacity of hepatocytes with a decrease in binucleated liver cells. In cytochrome gene expression, the mRNA level increased in CYP2E1 isoenzyme and reduced CYP1A2 expression. This study indicated that early treatment is necessary to mitigate APAP-induced acute liver injury, and alternative therapies capable of controlling the progression of intoxication in the liver are needed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Hepática Induzida por Substâncias e Drogas / Acetaminofen Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Hepática Induzida por Substâncias e Drogas / Acetaminofen Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article