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Endothelial cell-derived fibroblast growth factor-18 regulates ovarian function in sheep.
Estienne, Anthony; Relav, Lauriane; Benkoura, Morad; Monniaux, Danielle; Morin, Fanny; Fabre, Stéphane; Price, Christopher A.
Afiliação
  • Estienne A; Département de biomédecine vétérinaire, Centre de recherche en reproduction et fertilité (CRRF), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
  • Relav L; Physiologie Animale et Système d'Elevage, UMR Physiologie de la Reproduction et des Comportements, INRAE Tours, Nouzilly, France.
  • Benkoura M; Département de biomédecine vétérinaire, Centre de recherche en reproduction et fertilité (CRRF), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
  • Monniaux D; Département de biomédecine vétérinaire, Centre de recherche en reproduction et fertilité (CRRF), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
  • Morin F; Physiologie Animale et Système d'Elevage, UMR Physiologie de la Reproduction et des Comportements, INRAE Tours, Nouzilly, France.
  • Fabre S; Département de biomédecine vétérinaire, Centre de recherche en reproduction et fertilité (CRRF), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
  • Price CA; GenPhySE, Université de Toulouse, INRAE, ENVT, Castanet-Tolosan, France.
J Cell Physiol ; 237(5): 2528-2538, 2022 05.
Article em En | MEDLINE | ID: mdl-35315069
ABSTRACT
Increasing the efficiency of farm animal reproduction is necessary to reduce the environmental impact of food production systems. One approach is to increase the number of healthy eggs (oocytes) produced per female for fertilization, thus it is important to understand factors that decrease oocyte health. One paracrine factor that decreases ovarian follicle growth is fibroblast growth factor 18 (FGF18) secreted by cells in the theca layer of the ovarian follicle, however the factors that regulate FGF18 secretion are unknown. In this study we hypothesized that FGF18 secretion is controled by intrafollicular factors and is linked to fertility, which we tested by using cell culture and sheep genetic models in vivo. Separation of theca cell populations revealed that FGF18 messenger RNA (mRNA) is located mainly in thecal endothelial rather than endocrine cells, and immunohistochemistry localized FGF18 protein to microvessels in the theca layer in situ. Culture of ovine theca-derived endothelial cells was used to demonstrate stimulation of FGF18 mRNA and protein abundance by bone morphogenetic protein 4 (BMP4), a growth factor derived from theca endocrine cells. Taking advantage of a sheep genetic model, we demonstrate reduced ovarian and peripheral FGF18 concentrations in the hyperprolific Booroola ewe harboring the FecBB mutation in BMPR1B. These data suggest a novel control of fertility by follicular endothelial cells, in which theca endocrine cells secrete BMP4 that stimulates the secretion of FGF18 from thecal endothelial cells, which in turn diffuses into the granulosa cell layer and promotes apoptosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Tecais / Células Endoteliais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Tecais / Células Endoteliais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article