Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts.
Chem Biodivers
; 19(5): e202200047, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-35316577
ABSTRACT
Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6â
mg/g) was its main constituent. Extract inâ
vitro exhibited notable total antioxidant activity (FRAP value=1.0 µmol Fe2+ /mg), and scavenging of DPPH (SC50 =62.5â
µg/ml) and ⢠OH radicals (49.5 % at 20â
µg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100â
µg/ml), and reduced spasmogenic effect of KCl (80â
mm) to 44.4 % (at 60â
µg/ml) and of highest applied concentration of ACh to 26.3 % (at 120â
µg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100â
mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20â
mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ferula
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article