Your browser doesn't support javascript.
loading
Activation CRF-R2 augments cerebellar climbing fiber-Purkinje cell synaptic transmission via presynaptic PKA pathway in mice.
Cui, Li-Na; Zhang, Xu-Dong; Xu, Ying-Han; Li, Bo-Yang; Qiu, De-Lai; Chu, Chun-Ping.
Afiliação
  • Cui LN; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, Jilin Province, China; Department of Acupuncture, Affiliated Hospital of Yanbian University, Yanji, Jilin Province, China.
  • Zhang XD; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, Jilin Province, China; Department of Acupuncture, Affiliated Hospital of Yanbian University, Yanji, Jilin Province, China.
  • Xu YH; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, Jilin Province, China; Department of Acupuncture, Affiliated Hospital of Yanbian University, Yanji, Jilin Province, China.
  • Li BY; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, Jilin Province, China; Department of Acupuncture, Affiliated Hospital of Yanbian University, Yanji, Jilin Province, China.
  • Qiu DL; Department of Physiology, College of Basic Medicine, Jilin Medical University, Jilin City, Jilin Province, China. Electronic address: dlqiu@jlmu.edu.cn.
  • Chu CP; Department of Physiology, College of Basic Medicine, Jilin Medical University, Jilin City, Jilin Province, China. Electronic address: cpchu@ybu.edu.cn.
Neurosci Lett ; 777: 136584, 2022 04 23.
Article em En | MEDLINE | ID: mdl-35318076
Corticotropin releasing factor (CRF) type 2 receptor (CRF-R2) is present in climbing fiber (CF) afferents, which involves in modulating the CF-Purkinje cell (PC) synaptic transmission in cerebellar cortex. However, the role of CRF-R2 in regulating CF-PC synaptic transmission is unclear. We here investigate the role of CRF-R2 in modulating PC complex spikes (CSs) activity and CF-PC synaptic transmission using electrophysiological recording techniques and pharmacological methods. Cerebellar surface application of a selective CRF-R2 agonist, urocortin III (UCN III; 300 nM) induced an enhancement of CSs activity, which expressed an increase in number of CSs spikelets and pause of simple spike firing of cerebellar PCs in urethane anesthetized mice. The CSs activity was also enhanced by CRF (300 nM) in the presence of CRF-R1 antagonist, which was abolished by CRF-R2 antagonist. Under in vitro conditions, bath application of UCN III increased CF-PC synaptic transmission, which exhibited a time-dependent increase in amplitude of excitatory postsynaptic currents (EPSCs), accompanied by a decrease in paired-pulse ratio (PPR). In addition, bath application of CRF (100 nM) induced an increase in amplitude of EPSCs and a decrease in PPR in the absence of CRF-R1 activity. UCN-induced enhancement of CF-PC synaptic transmission was abolished by bath application of protein kinase A (PKA) inhibitor, KT5720 (100 nM), but it was not prevented by inhibiting intracellular PKA with PKI (5 µM). These results indicate that activation of CRF-R2 augments CF-PC synaptic transmission through a presynaptic PKA signaling pathway in the mouse cerebellar cortex.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Purkinje / Hormônio Liberador da Corticotropina Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Purkinje / Hormônio Liberador da Corticotropina Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article