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Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach.
Liang, Mindong; Liu, Leshi; Xu, Fei; Zeng, Xiaoqian; Wang, Runjun; Yang, Jinling; Wang, Weishan; Karthik, Loganathan; Liu, Jiakun; Yang, Zhiheng; Zhu, Guoliang; Wang, Shuliu; Bai, Linquan; Tong, Yaojun; Liu, Xueting; Wu, Min; Zhang, Li-Xin; Tan, Gao-Yi.
Afiliação
  • Liang M; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Liu L; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Xu F; Institute of Pharmaceutical Biotechnology and Department of Gastroenterology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • Zeng X; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Wang R; Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou 313000, China.
  • Yang J; Institute of Pharmaceutical Biotechnology and Department of Gastroenterology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • Wang W; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  • Karthik L; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Liu J; Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • Yang Z; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Zhu G; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Wang S; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Bai L; State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Tong Y; State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Liu X; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Wu M; College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
  • Zhang LX; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
  • Tan GY; State Key Laboratory of Bioreactor Engineering and School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
Nucleic Acids Res ; 50(6): 3581-3592, 2022 04 08.
Article em En | MEDLINE | ID: mdl-35323947
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Produtos Biológicos Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Produtos Biológicos Idioma: En Ano de publicação: 2022 Tipo de documento: Article