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Combined exposure to fine particulate matter and high glucose aggravates endothelial damage by increasing inflammation and mitophagy: the involvement of vitamin D.
Lai, Tsai-Chun; Chen, Yu-Chen; Cheng, Hui-Hua; Lee, Tzu-Lin; Tsai, Jaw-Shiun; Lee, I-Ta; Peng, Kuo-Ti; Lee, Chiang-Wen; Hsu, Lee-Fen; Chen, Yuh-Lien.
Afiliação
  • Lai TC; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, 100233, Taiwan.
  • Chen YC; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, 100233, Taiwan.
  • Cheng HH; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, 100233, Taiwan.
  • Lee TL; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, 100233, Taiwan.
  • Tsai JS; Department of Family Medicine, National Taiwan University Hospital, Taipei, 100225, Taiwan.
  • Lee IT; Center for Complementary and Integrated Medicine, National Taiwan University Hospital, Taipei, 100225, Taiwan.
  • Peng KT; School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, 110301, Taiwan.
  • Lee CW; Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Puzi City, Chiayi County, 613016, Taiwan.
  • Hsu LF; College of Medicine, Chang Gung University, Guishan District, Taoyuan City, 333323, Taiwan.
  • Chen YL; Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Puzi City, Chiayi County, 613016, Taiwan. cwlee@gw.cgust.edu.tw.
Part Fibre Toxicol ; 19(1): 25, 2022 03 29.
Article em En | MEDLINE | ID: mdl-35351169
BACKGROUND: Cardiovascular diseases (CVDs) are related to particulate matter (PM2.5) exposure. Researchers have not clearly determined whether hyperglycemia, a hallmark of diabetes, exacerbates PM2.5-induced endothelial damage. Thus, this study aimed to investigate the combined effects of PM2.5 and high glucose on endothelial damage. RESULTS: Here, we treated human umbilical vein endothelial cells (HUVECs) with 30 mM high glucose and 50 µg/mL PM (HG + PM) to simulate endothelial cells exposed to hyperglycemia and air pollution. First, we showed that HUVECs exposed to PM under high glucose conditions exhibited significant increases in cell damage and apoptosis compared with HUVECs exposed to PM or HG alone. In addition, PM significantly increased the production of reactive oxygen species (ROS) in HUVECs and mitochondria treated with HG and decreased the expression of superoxide dismutase 1 (SOD1), a free radical scavenging enzyme. The coexposure group exhibited significantly increased ROS production in cells and mitochondria, a lower mitochondrial membrane potential, and increased levels of the autophagy-related proteins p62, microtubule-associated protein 1 light chain 3ß (LC3B), and mitophagy-related protein BCL2 interacting protein 3 (Bnip3). Moreover, autophagosome-like structures were observed in the HG + PM group using transmission electron microscopy. The expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were also increased through the JNK/p38 signaling pathway in the HG + PM group. As a ROS scavenger, vitamin D treatment effectively protected cells under HG and PM conditions by increasing cell viability, reducing mitochondrial ROS production, and suppressing the formation of mitophagy and inflammation. Furthermore, diabetes was induced in mice by administering streptozotocin (STZ). Mice were treated with PM by intratracheal injection. Vitamin D effectively alleviated oxidative stress, mitophagy, and inflammation in the aortas of mice treated with STZ and PM. CONCLUSION: Taken together, simultaneous exposure to PM and high glucose exerts significant harmful effects on endothelial cells by inducing ROS production, mitophagy, and inflammation, while vitamin D reverses these effects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina D / Mitofagia Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina D / Mitofagia Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article