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Klebsiella oxytoca: an efficient pyrene-degrading bacterial strain isolated from petroleum-contaminated soil.
Alfaify, Abdulkhaleg M; Mir, Mushtaq Ahmad; Alrumman, Sulaiman A.
Afiliação
  • Alfaify AM; Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
  • Mir MA; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P. O. Box 3665, Abha, 61421, Saudi Arabia. mmir@kku.edu.sa.
  • Alrumman SA; Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
Arch Microbiol ; 204(5): 248, 2022 Apr 09.
Article em En | MEDLINE | ID: mdl-35397012
Polycyclic aromatic hydrocarbons (PAHs) are the hazardous xenobiotic agents of oil production. One of the methods to eliminate hazardous compounds is bioremediation, which is the most efficient and cost-effective method to eliminate the harmful byproducts of crude petroleum processing. In this study, five pure bacterial isolates were isolated from petroleum-contaminated soil, four of which showed a robust growth on the PAH pyrene, as a sole carbon source. Various methods viz mass spectroscopy, biochemical assays, and 16S RNA sequencing employed to identify the isolates ascertained the consistent identification of Klebsiella oxytoca by all three methods. Scanning electron microscopy and Gram staining further demonstrated the characterization of the K. oxytoca. High-performance liquid chromatography of the culture supernatant of K. oxytoca grown in pyrene containing media showed that the cells started utilizing pyrene from the 6th day onwards and by the 12th day of growth, 70% of the pyrene was completely degraded. A genome search for the genes predicted to be involved in pyrene degradation using Kyoto Encyclopedia of Genes and Genomes (KEGG) confirmed their presence in the genome of K. oxytoca. These results suggest that K. oxytoca would be a suitable candidate for removing soil aromatic hydrocarbons.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Poluentes do Solo / Petróleo Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Poluentes do Solo / Petróleo Idioma: En Ano de publicação: 2022 Tipo de documento: Article