Your browser doesn't support javascript.
loading
Psoralen mapping reveals a bacterial genome supercoiling landscape dominated by transcription.
Visser, Bryan J; Sharma, Sonum; Chen, Po J; McMullin, Anna B; Bates, Maia L; Bates, David.
Afiliação
  • Visser BJ; Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, TX 77030, USA.
  • Sharma S; Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
  • Chen PJ; Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
  • McMullin AB; Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
  • Bates ML; Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
  • Bates D; Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, TX 77030, USA.
Nucleic Acids Res ; 50(8): 4436-4449, 2022 05 06.
Article em En | MEDLINE | ID: mdl-35420137
DNA supercoiling is a key regulator of all DNA metabolic processes including replication, transcription, and recombination, yet a reliable genomic assay for supercoiling is lacking. Here, we present a robust and flexible method (Psora-seq) to measure whole-genome supercoiling at high resolution. Using this tool in Escherichia coli, we observe a supercoiling landscape that is well correlated to transcription. Supercoiling twin-domains generated by RNA polymerase complexes span 25 kb in each direction - an order of magnitude farther than previous measurements in any organism. Thus, ribosomal and many other highly expressed genes strongly affect the topology of about 40 neighboring genes each, creating highly integrated gene circuits. Genomic patterns of supercoiling revealed by Psora-seq could be aptly predicted from modeling based on gene expression levels alone, indicating that transcription is the major determinant of chromosome supercoiling. Large-scale supercoiling patterns were highly symmetrical between left and right chromosome arms (replichores), indicating that DNA replication also strongly influences supercoiling. Skew in the axis of symmetry from the natural ori-ter axis supports previous indications that the rightward replication fork is delayed several minutes after initiation. Implications of supercoiling on DNA replication and chromosome domain structure are discussed.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Super-Helicoidal / Ficusina Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Super-Helicoidal / Ficusina Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article