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Ultrafast Doppler imaging and ultrasound localization microscopy reveal the complexity of vascular rearrangement in chronic spinal lesion.
Beliard, Benoit; Ahmanna, Chaimae; Tiran, Elodie; Kanté, Kadia; Deffieux, Thomas; Tanter, Mickael; Nothias, Fatiha; Soares, Sylvia; Pezet, Sophie.
Afiliação
  • Beliard B; Institute of Physics for Medicine Paris, Inserm U1273, ESPCI PSL Paris, CNRS UMR8361, PSL Research University - Paris, 17 rue Moreau, 75012, Paris, France.
  • Ahmanna C; Neuroscience Paris Seine NPS, CNRS UMR8246, INSERM U1130, UM119, Institut de Biologie Paris Seine IBPS, Sorbonne Université Sciences, Campus UPMC, 75005, Paris, France.
  • Tiran E; Institute of Physics for Medicine Paris, Inserm U1273, ESPCI PSL Paris, CNRS UMR8361, PSL Research University - Paris, 17 rue Moreau, 75012, Paris, France.
  • Kanté K; Neuroscience Paris Seine NPS, CNRS UMR8246, INSERM U1130, UM119, Institut de Biologie Paris Seine IBPS, Sorbonne Université Sciences, Campus UPMC, 75005, Paris, France.
  • Deffieux T; Institute of Physics for Medicine Paris, Inserm U1273, ESPCI PSL Paris, CNRS UMR8361, PSL Research University - Paris, 17 rue Moreau, 75012, Paris, France.
  • Tanter M; Institute of Physics for Medicine Paris, Inserm U1273, ESPCI PSL Paris, CNRS UMR8361, PSL Research University - Paris, 17 rue Moreau, 75012, Paris, France.
  • Nothias F; Neuroscience Paris Seine NPS, CNRS UMR8246, INSERM U1130, UM119, Institut de Biologie Paris Seine IBPS, Sorbonne Université Sciences, Campus UPMC, 75005, Paris, France.
  • Soares S; Neuroscience Paris Seine NPS, CNRS UMR8246, INSERM U1130, UM119, Institut de Biologie Paris Seine IBPS, Sorbonne Université Sciences, Campus UPMC, 75005, Paris, France. sylvia.soares@upmc.fr.
  • Pezet S; Institute of Physics for Medicine Paris, Inserm U1273, ESPCI PSL Paris, CNRS UMR8361, PSL Research University - Paris, 17 rue Moreau, 75012, Paris, France. sophie.pezet@espci.fr.
Sci Rep ; 12(1): 6574, 2022 04 21.
Article em En | MEDLINE | ID: mdl-35449222
ABSTRACT
Acute spinal cord injury (SCI) leads to severe damage to the microvascular network. The process of spontaneous repair is accompanied by formation of new blood vessels; their functionality, however, presumably very important for functional recovery, has never been clearly established, as most studies so far used fixed tissues. Here, combining ultrafast Doppler imaging and ultrasound localization microscopy (ULM) on the same animals, we proceeded at a detailed analysis of structural and functional vascular alterations associated with the establishment of chronic SCI, both at macroscopic and microscopic scales. Using a standardized animal model of SCI, our results demonstrate striking hemodynamic alterations in several subparts of the spinal cord a reduced blood velocity in the lesion site, and an asymmetrical hypoperfusion caudal but not rostral to the lesion. In addition, the worsening of many evaluated parameters at later time points suggests that the neoformed vascular network is not yet fully operational, and reveals ULM as an efficient in vivo readout for spinal cord vascular alterations. Finally, we show statistical correlations between the diverse biomarkers of vascular dysfunction and SCI severity. The imaging modality developed here will allow evaluating recovery of vascular function over time in pre-clinical models of SCI. Also, used on SCI patients in combination with other quantitative markers of neural tissue damage, it may help classifying lesion severity and predict possible treatment outcomes in patients.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Microscopia Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Microscopia Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article