First Human Cell Experiments With FLASH Carbon Ions.
Anticancer Res
; 42(5): 2469-2477, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-35489744
ABSTRACT
BACKGROUND/AIM:
This study aimed to establish a setup for ultra-high-dose-rate (FLASH) carbon-ion irradiation, and to conduct the first human cell experiments using FLASH carbon ions. MATERIALS ANDMETHODS:
A system for FLASH carbon-ion irradiation (1-3 Gy at 13 or 50 keV/µm) was developed. The growth and senescence of HFL1 lung fibroblasts were assessed by crystal violet staining assays and senescence-associated ß-galactosidase staining, respectively. Survival of HSGc-C5 cancer cells was assessed by clonogenic assays.RESULTS:
The dose rates of carbon ions ranged from 96-195 Gy/s, meeting the definition of FLASH. With both 13 and 50 keV/µm beams, no FLASH sparing effect was observed on the growth suppression and senescence of HFL1 cells, nor on the survival of HSGc-C5 cells.CONCLUSION:
We successfully conducted the first human cell experiments with FLASH carbon ions. No FLASH effect was observed under the conditions examined.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbono
/
Radioterapia com Íons Pesados
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article