An Extracorporeal Plasma Filtration Column with Specific Binding to Dengue Virions.
Blood Purif
; 52(1): 60-67, 2023.
Article
em En
| MEDLINE
| ID: mdl-35512639
ABSTRACT
INTRODUCTION:
Dengue infection is a significant public health concern that no specific treatment is available. Extracorporeal plasmapheresis or plasma filtration is a treatment option for severe cases with complications. However, the commercial adsorption devices mainly contained size-exclusive porous beads to adsorb the plasma proteins nonselectively.METHODS:
We developed a 150 simulated circuit for dengue virus-specific adsorption using a flavivirus-specific (4G2) antibody entrapped into the alginate bead.RESULTS:
The reduction ratios of the viral titer after 3 h of continuous run were 63.00 ± 1.21%, and 93.97 ± 1.27% measured by reverse transcription qPCR, and plaque titration, respectively. No specific adsorption was observed with Enterovirus A71 or Escherichia coli bacteria.CONCLUSION:
This study is a proof-of-concept for the potential use of a dengue virus-specific adsorption column in the 150 simulated circuit. The system could be applied to various clinical platforms by substituting target-specific antibodies.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dengue
/
Vírus da Dengue
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article