Mutagenesis analysis of GMN motif in Arabidopsis thaliana Mg2+ transporter MRS2-1.
Biosci Biotechnol Biochem
; 86(7): 870-874, 2022 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-35524690
ABSTRACT
Magnesium is an important nutrient for plants, but much is still unknown about plant Mg2+ transporters. Combining with the structural prediction of AlphaFold2, we used mutagenesis and 28Mg uptake assay to study the highly conserved "GMN" motif of Arabidopsis thaliana MRS2-1 (AtMRS2-1) transporter. We demonstrated that the glycine and methionine in GMN motif are essential for AtMRS2-1 to transport Mg2+.
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01-internacional
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MEDLINE
Assunto principal:
Arabidopsis
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Proteínas de Transporte de Cátions
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Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article