Structural characterization and biological evaluation of a new O-acetyl-1,4-linked-ß-d-mannan possessed potential application in hydrophilic polymer materials from Dendrobium devonianum.
Int J Biol Macromol
; 213: 328-338, 2022 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-35594938
ABSTRACT
To explore the active polysaccharides from Dendrobium devonianum, a novel O-acetylmannan (DDP-1) with molecular weight of 117 kDa was isolated from D. devonianum. The chemical and instrumental analysis indicated that the DDP-1 was a homopolysaccharide containing a backbone chain composed of â4)-ß-d-Manp-(1 â (71.4%) residue with internal â4)-2-O-acetyl-ß-d-Manp-(1 â (14.2%), â4)-3-O-acetyl-ß-d-Manp-(1 â (7.1%), and non-reducing end ß-d-Manp-(1 â (7.3%) residues. Anticancer assay in vitro revealed that DDP-1 had anticancer activity against the growth of HepG2 and MCF-7 cancer cells. Moreover, cytokine secretion assays also presented that DDP-1 can promote cytokine production of TNF-α and IL-6 in THP-1 macrophage stimulated by PMA. Finally, the effects of isolation and purification on the microstructure of DDP-1 was studied by scanning electron microscope. The morphological features of DDP-1 indicated that DDP-1 hold high potential application in hydrophilic polymer materials.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dendrobium
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article