Your browser doesn't support javascript.
loading
Dexmedetomidine Leads to the Mitigation of Myocardial Ischemia/Reperfusion-Induced Acute Lung Injury in Diabetic Rats Via Modulation of Hypoxia-Inducible Factor-1α.
Chen, Siyu; Wu, Jianjiang; Yang, Long; Tailaiti, Taiwangu; Zou, Tiantian; Huan, Yidan; Wang, Jiang.
Afiliação
  • Chen S; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Wu J; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Yang L; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Tailaiti T; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Zou T; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Huan Y; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
  • Wang J; The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
Braz J Cardiovasc Surg ; 37(3): 370-379, 2022 05 23.
Article em En | MEDLINE | ID: mdl-35605218
INTRODUCTION: The objective of this study is to investigate the protective mechanism of dexmedetomidine (Dex) in myocardial ischemia/reperfusion (MIR)-induced acute lung injury (ALI) of diabetic rats by inhibiting hypoxia-inducible factor-1α (HIF-1α). METHODS: Initially, healthy male Sprague Dawley rats were treated with streptozocin to induce diabetes. Then, three weeks after the induction, Dex or lentiviral vector (LV)-HIF-1α was injected into the rats 30 minutes prior to the MIR modeling. After four weeks, lung tissues were harvested for pathological changes observation and the wet/dry weight (W/D) ratio determination. Afterwards, oxidative stress indicators and pro-inflammatory factors were measured. In addition, HIF-1α expression was assessed by immunohistochemistry and western blot analysis. RESULTS: Dex could suppress inflammatory cell infiltration, improve lung tissue structure, reduce pathological score and the W/D ratio, and block oxidative stress and inflammatory response in MIR-induced ALI of diabetic rats. Besides, Dex could also inhibit HIF-1α expression. Moreover, Dex + LV-HIF-1α reversed the protective role of Dex on diabetic MIR-induced ALI. CONCLUSION: Our study has made it clear that Dex inhibited the upregulation of HIF-1α in diabetic MIR-induced ALI, and thus protect lung functions by quenching the accumulation of oxygen radical and reducing lung inflammatory response.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Dexmedetomidina / Diabetes Mellitus Experimental / Lesão Pulmonar Aguda Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Dexmedetomidina / Diabetes Mellitus Experimental / Lesão Pulmonar Aguda Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2022 Tipo de documento: Article