Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics.

Piyadasa, Hadeesha; Lao, Ying; Krokhin, Oleg; Mookherjee, Neeloffer

Piyadasa, Hadeesha; Lao, Ying; Krokhin, Oleg; Mookherjee, Neeloffer.

Afiliação

Piyadasa H; Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.
Lao Y; Department of Immunology, University of Manitoba, Winnipeg, MB, Canada.
Krokhin O; Department of Pathology, School of Medicine, Stanford University, Palo Alto, CA, USA.
Mookherjee N; Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.

Methods Mol Biol ; 2456: 53-62, 2022.

Article em En | MEDLINE | ID: mdl-35612734

ABSTRACT

ABSTRACT

Mass spectrometry (MS) is a routinely used approach to characterize global protein profile in various biological samples. Here we describe rodent lung tissue homogenization, sample preparation, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for shotgun proteomics.

Assuntos

Proteômica; Espectrometria de Massas em Tandem; Animais; Cromatografia Líquida/métodos; Pulmão; Proteômica/métodos; Roedores

Palavras-chave

Mass spectrometry; Rodent lungs; Sample preparation; Shotgun proteomics

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Espectrometria de Massas em Tandem Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google