Your browser doesn't support javascript.
loading
The Exploration of miRNAs From Porcine Fallopian Tube Stem Cells on Porcine Oocytes.
Fu, Tzu-Yen; Wang, Shu-Hsuan; Lin, Tzu-Yi; Shen, Perng-Chih; Chang, Shen-Chang; Lin, Yu-Han; Chou, Chih-Jen; Yu, Yu-Hsiang; Yang, Kuo-Tai; Huang, Chao-Wei; Shaw, Steven W; Peng, Shao-Yu.
Afiliação
  • Fu TY; Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Wang SH; Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Lin TY; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • Shen PC; Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Chang SC; Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Executive Yuan, Pingtung, Taiwan.
  • Lin YH; Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Chou CJ; Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
  • Yu YH; Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
  • Yang KT; Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Huang CW; Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Shaw SW; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • Peng SY; Department of Obstetrics and Gynecology, Taipei Chang Gung Memorial Hospital, Taipei, Taiwan.
Front Vet Sci ; 9: 869217, 2022.
Article em En | MEDLINE | ID: mdl-35615247
Fallopian tube is essential to fertilization and embryonic development. Extracellular vesicles (EVs) from Fallopian tube containing biological regulatory factors, such as lipids, proteins and microRNAs (miRNAs) serve as the key role. At present, studies on oocytes from porcine oviduct and components from EVs remain limited. We aim to explore the effect of EVs secreted by porcine fallopian tube stem cells (PFTSCs) on oocyte. When the fifth-generation PFTSCs reached 80-90% of confluency, the pig in vitro maturation medium was utilized, and the conditioned medium collected for oocyte incubations. To realize the functions of EVs, several proteins were used to determine whether extracted EVs were cell-free. Field emission scanning electron microscope and nanoparticle tracking analyzer were used to observe the morphology. By next generation sequencing, 267 miRNAs were identified, and those with higher expression were selected to analyze the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment maps. The selected miR-152-3p, miR-148a-3p, miR-320a-3p, let-7f-5p, and miR-22-3p, were predicted to target Cepb1 gene affecting MAPK pathway. Of the five miRNAs, miR-320a-3p showed significant difference in maturation rate in vitro maturation. The blastocyst rate of pig embryos was also significantly enhanced by adding 50 nM miR-320a-3p. In vitro culture with miR-320a-3p, the blastocyst rate was significantly higher, but the cleavage rate and cell numbers were not. The CM of PFTSCs effectively improves porcine oocyte development. The miRNAs in EVs are sequenced and identified. miR-320a-3p not only helps the maturation, but also increases the blastocyst rates.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article