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Dual-emitting BP-CdTe QDs coupled with dual-function moderator TiO2 NSs for electrochemiluminescence ratio bioassay.
Zhao, Jinwen; Zhou, Yuting; He, Ying; Tan, Xingrong; Yuan, Ruo; Chen, Shihong.
Afiliação
  • Zhao J; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
  • Zhou Y; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
  • He Y; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
  • Tan X; Department of Endocrinology, 9 th People's Hospital of Chongqing, Chongqing, 400700, PR China. Electronic address: tanxingrong@yahoo.com.
  • Yuan R; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
  • Chen S; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address: cshong@swu.edu.cn.
Biosens Bioelectron ; 212: 114420, 2022 Sep 15.
Article em En | MEDLINE | ID: mdl-35635968
The limitation of the selection of dual-emitting luminophor and dual-function moderator makes the construction of single-luminophor-based electrochemiluminescence (ECL) ratio strategy extremely challenging. This work developed black phosphorus nanosheet loaded with CdTe quantum dots (BP-CdTe QDs) as dual-emitting luminophor, and TiO2 nanosheets (NSs) as dual-function moderator to construct single-luminophor-based ECL ratio biosensor for amyloid-ß protein (Aß) detection. The BP-CdTe QDs modified glassy carbon electrode (GCE) firstly captured the anti-Aß1, and then the target Aß. Finally, the secondary antibody composites containing N,N-diethylethylenediamine (DEDA) and TiO2 NSs were captured on the electrode surface. DEDA in the secondary antibody composites and S2O82- added in the detection solution served as the anodic and cathodic co-reactants, respectively, and TiO2 NSs as co-reaction accelerator can simultaneously enhance two emissions. The increase of target concentration made the two signals show different increasing trend, thus achieving a sensitive ratio detection of target Aß with the detection limit of 21 fg/mL. The dual-emitting BP-CdTe QDs coupled with dual-function moderator TiO2 NSs provide an attractive single-luminophor-based ECL ratio platform for bioassay, which can not only be applied to the detection of Aß, but also to the detection of other disease biomarkers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Compostos de Cádmio / Pontos Quânticos Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Compostos de Cádmio / Pontos Quânticos Idioma: En Ano de publicação: 2022 Tipo de documento: Article