Dual-emitting BP-CdTe QDs coupled with dual-function moderator TiO2 NSs for electrochemiluminescence ratio bioassay.
Biosens Bioelectron
; 212: 114420, 2022 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-35635968
The limitation of the selection of dual-emitting luminophor and dual-function moderator makes the construction of single-luminophor-based electrochemiluminescence (ECL) ratio strategy extremely challenging. This work developed black phosphorus nanosheet loaded with CdTe quantum dots (BP-CdTe QDs) as dual-emitting luminophor, and TiO2 nanosheets (NSs) as dual-function moderator to construct single-luminophor-based ECL ratio biosensor for amyloid-ß protein (Aß) detection. The BP-CdTe QDs modified glassy carbon electrode (GCE) firstly captured the anti-Aß1, and then the target Aß. Finally, the secondary antibody composites containing N,N-diethylethylenediamine (DEDA) and TiO2 NSs were captured on the electrode surface. DEDA in the secondary antibody composites and S2O82- added in the detection solution served as the anodic and cathodic co-reactants, respectively, and TiO2 NSs as co-reaction accelerator can simultaneously enhance two emissions. The increase of target concentration made the two signals show different increasing trend, thus achieving a sensitive ratio detection of target Aß with the detection limit of 21 fg/mL. The dual-emitting BP-CdTe QDs coupled with dual-function moderator TiO2 NSs provide an attractive single-luminophor-based ECL ratio platform for bioassay, which can not only be applied to the detection of Aß, but also to the detection of other disease biomarkers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Compostos de Cádmio
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Pontos Quânticos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article