Preparation of a multifunctional photoactivated prodrug on a streptavidin scaffold bearing a DNA aptamer.
Bioorg Med Chem Lett
; 71: 128819, 2022 09 01.
Article
em En
| MEDLINE
| ID: mdl-35643261
ABSTRACT
Prodrugs that present strong cytotoxicity toward specific cells have been utilized for cell-type selection and purification. In this study, we designed and prepared a multifunctional, photoactivated prodrug based on a streptavidin scaffold. Biotin-labeled DNA aptamer that recognizes the membrane antigen EpCAM, and biotin-labeled photoactivated prodrug bearing the antitumor camptothecin, were prepared. Both molecules were linked to the streptavidin scaffold by simple mixing. The resulting prodrug bound to the EpCAM-overexpressing SK-BR-3 target cells and showed cytotoxic effects upon photoirradiation, corresponding to cytotoxic drug release.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pró-Fármacos
/
Aptâmeros de Nucleotídeos
/
Antineoplásicos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article